Table 1. Intracellular concentration of Ca2+ ([Ca2+]i nM).
days | Proliferative medium (PM) | Osteogenic medium (OM) | ||
---|---|---|---|---|
TiO2 | TiO2/PEMF+ | TiO2 | TiO2/PEMF+ | |
3 | 63 ± 5 (§) (**) | 87 ± 2(# #) (***) | 105 ± 2(**) | 155±2 (***) (# #) |
28 | 69 ± 7 (§) (**) | 192 ± 8(# #) | 299 ± 11(**) | 293 ± 9(**) |
Intracellular concentration has been analysed performing Ca2+ Fura-2 measurements in hBM-MSCs. The trend of different cell populations growing adherent to TiO2 was monitored after 3 days and at the end of the culture (28 days). The data, representing the results of three measurements in two separated experiments, are expressed as the mean ± SEM (** p < 0.01 vs. cultures grown on TiO2 (§)). Similar values were also obtained in ref [12] even though from different experiments and at different checkpoints. Data represent the results of three sets of independent experiments and are expressed as mean ± SEM (** p < 0.01 and *** p< 0.001 vs. cultures grown on glass in PM; ## p < 0.01 vs. cultures grown on TiO2).