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. 2018 Jun 14;13(6):e0199046. doi: 10.1371/journal.pone.0199046

Table 1. Intracellular concentration of Ca2+ ([Ca2+]i nM).

days Proliferative medium (PM) Osteogenic medium (OM)
TiO2 TiO2/PEMF+ TiO2 TiO2/PEMF+
3 63 ± 5 (§) (**) 87 ± 2(# #) (***) 105 ± 2(**) 155±2 (***) (# #)
28 69 ± 7 (§) (**) 192 ± 8(# #) 299 ± 11(**) 293 ± 9(**)

Intracellular concentration has been analysed performing Ca2+ Fura-2 measurements in hBM-MSCs. The trend of different cell populations growing adherent to TiO2 was monitored after 3 days and at the end of the culture (28 days). The data, representing the results of three measurements in two separated experiments, are expressed as the mean ± SEM (** p < 0.01 vs. cultures grown on TiO2 (§)). Similar values were also obtained in ref [12] even though from different experiments and at different checkpoints. Data represent the results of three sets of independent experiments and are expressed as mean ± SEM (** p < 0.01 and *** p< 0.001 vs. cultures grown on glass in PM; ## p < 0.01 vs. cultures grown on TiO2).