Fig 3. Isotype and avidity of anti-FliC IgG in adult and infant mice after immunization with S. Enteritidis COPS:FliC alone or formulated with different adjuvants.
(A) The avidity of anti-FliC IgG in sera taken after each immunization (n = 8–10) was determined by sensitivity to urea treatment in an ELISA format. (B) Post 3rd immunization FliC-specific serum IgG1 and IgG2b titers were determined by ELISA (n = 9–10). The ratio of the two titers is plotted. Each point represents an individual mouse. Bars represent the median for adults (grey) and infants (white) that was compared using a two-tailed Mann-Whitney U test. Adjustments for multiple comparisons were not made. P-values ≤ 0.05 were considered to be statistically significant; ns, not significant. *P ≤ 0.05; **P ≤ 0.005; ***P ≤ 0.0005 for indicated comparisons.