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. 2018 Jun 14;13(6):e0197971. doi: 10.1371/journal.pone.0197971

Fig 2. Heat stability of soluble recombinant Sola l 4.02.

Fig 2

(A) SDS-PAGE and (B) Western-Blot analysis of purified pooled elution fractions of the recombinant Sola l 4.02 protein heated for 10, 20, 30, 60 and 90 min at 99 °C. Untreated protein served as control (0). SDS-PAGE was performed under reducing conditions. Coomassie Brilliant Blue G250 was used for protein staining. For Western blot analysis specific polyclonal Sola l 4-antibody was used. M: PageRuler Prestained Protein Ladder.