Figure 3. Effect of HOTAIR on UVB-induced cell injury; A, qRT-PCR revealed overexpression and suppression of HOTAIR in HaCaT cells after transfection assay; B, Cell viability assay revealed suppression of HOTAIR alleviated UVB-induced low cell viability and overexpression of HOTAIR further promoted UVB-induced inhibition of cell viability; C, Apoptosis assay revealed suppression of HOTAIR alleviated UVB-induced high cell apoptosis and overexpression of HOTAIR further promoted UVB-induced high cell apoptosis. D, qRT-PCR analysis showed that mRNA expression of Bcl-2 was increased after HOTAIR was silenced and decreased after HOTAIR was overexpressed. E, Western blotting analysis showed increased expression of Bax and decreased expression of Bcl-2 in HOTAIR overexpressed cells; F, ELISA results showed suppression of HOTAIR alleviated UVB-induced high expression of TNF-α and overexpression of HOTAIR further promoted UVB-induced high expression of TNF-α; G, ELISA results showed suppression of HOTAIR alleviated UVB-induced high expression of IL-6 and overexpression of HOTAIR further promoted UVB-induced high expression of IL-6; H, Western blotting analysis showed increased expression of TNF-α and IL-6 with HOTAIR overexpression. Data are reported as means±SD. *P<0.05; **P<0.01; ***P<0.001 (ANOVA). ns: P>0.05. UVB treatment time was 16 h. HOTAIR: HOX antisense intergenic RNA; UVB: ultraviolet B; qRT-PCR: quantitative real-time polymerase chain reaction; TNF-α: tumor necrosis factor-α; IL-6: interleukin-6.