Figure 6. Effect of HOTAIR and PKR on UVB-induced cell injury; A, Cell viability assay demonstrated overexpression of HOTAIR promoted UVB-induced cell viability inhibition by upregulation of PKR, and suppression of PKR reversed the results; B, Apoptosis assay showed overexpression of HOTAIR promoted UVB-induced cell apoptosis by upregulation of PKR, and suppression of PKR reversed the results; C, Western blotting analysis revealed overexpression of HOTAIR promoted the effects of UVB on apoptosis-related factors by upregulation of PKR, and suppression of PKR reversed the results; D and E, ELISA revealed overexpression of HOTAIR promoted UVB-induced high expression of TNF-α and IL-6 by upregulation of PKR, and suppression of PKR reversed the results; F, Western blotting analysis showed overexpression of HOTAIR promoted UVB-induced high expression of TNF-α and IL-6 by upregulation of PKR, and suppression of PKR reversed the results; G, Inhibiting expression of HOTAIR reversed the viability-inhibitory effect of PKR; H, Inhibiting expression of HOTAIR reversed the apoptosis-promoting effect of PKR; I, HOTAIR silence reversed the effects of PKR on apoptosis-related factors. Data are reported as means±SD. *P<0.05; **P<0.01 (ANOVA). UVB treatment time was 16 h. HOTAIR: HOX antisense intergenic RNA; PKR: RNA-dependent protein kinase; UVB: ultraviolet B; qRT-PCR: quantitative real-time polymerase chain reaction; TNF-α: tumor necrosis factor-α; IL-6: interleukin-6.
