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. 2018 Jun 4;14(6):e1007059. doi: 10.1371/journal.ppat.1007059

Fig 4. Tethering of T. cruzi RBPs to reporter mRNA.

Fig 4

(A) Scheme of the DNA construct cloned in the pTEX vector. See Methods section for details. (B) Normalization of reporter mRNA and luciferase activity values. See Methods section for details. RNA: normalized luciferase mRNA, P: normalized RBP protein, L: normalized luciferase activity, A: reporter mRNA abundance, T: reporter mRNA translation. (C) Trypanosoma cruzi epimastigotes were transfected with the reporter construct in which different RBPs (TcPABP1, TcUBP1, TcRBP4, TcZFP2 and TcZFP3) were artificially tethered to the 3´ UTR of the reporter gene. The results indicate folds over values obtained with GFP as the tethered protein. Folds higher than 2 were considered significative (*). Results are shown for 3 or 4 independent experiments.