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. 2018 Mar 22;30(5):1062–1076. doi: 10.1105/tpc.17.00845

Figure 2.

Figure 2.

PRU1 Expression Pattern.

(A) Analysis of PRU1 expression in wild-type plants using RT-qPCR. The wild-type plants were germinated and grown on MS medium for 7 d, and the roots and shoots were harvested separately for RNA extraction. Data are mean values of three biological replicates ± se.

(B) Analysis of PRU1 expression during Pi starvation using RT-qPCR. The 7-d-old wild-type seedlings were transferred to LP medium and then the roots were harvested at the indicated time points for RNA extraction. Data are mean values of three biological replicates ± se.

(C) Analysis of PRU1 expression during Pi starvation using RNA gel blot analysis. Seven-day-old wild-type seedlings were transferred to LP medium, and the roots were harvested at the indicated time points for RNA extraction. ACT7 and rRNA were used as loading controls.

(D) Analysis of PRU1 expression under nitrogen or sucrose deficiency using RT-qPCR. The 7-d-old wild-type seedlings were transferred to –N or –Suc medium, and then the roots were harvested at the indicated time points for RNA extraction. Data are mean values of three biological replicates ± se.