Figure 3.

Venn Diagrams Showing the Distribution of SUMOylated Proteins Purified from Wild-Type and siz1-2 Seedlings before and after Heat Stress.

Eight-day-old green seedlings were either kept at 22°C or heat stressed for 30 min at 37°C before tissue collection and SUMO1/SUMO2 conjugate purification.

(A) Overlaps of all SUMO1/SUMO2 conjugates detected in the wild-type or siz1-2 background exposed to heat stress (HS) versus control conditions (unstressed).

(B) Comparison of the total collection of SUMO1/SUMO2 conjugates in wild-type and siz1-2 seedlings after heat stress.

(C) Comparison of the abundant SUMO1/SUMO2 conjugates in wild-type or siz1-2 seedlings after heat stress. Abundant conjugates refer to those detected in three or more biological replicates in either wild-type or siz1-2 seedlings.

(D) Comparisons of the SUMO1/SUMO2 conjugates identified in this study with those previously described by Miller et al. (2010). The left diagram includes all SUMO1/SUMO2 conjugates detected in unstressed samples as well as samples exposed to heat and hydrogen peroxide stress. The right panel includes SUMO1/SUMO2 conjugates that were detected only in heat-stressed samples.