Localization and Functional Enrichments of SUMOylated Proteins from Wild-Type and siz1-2 Seedlings.
(A) Pie charts illustrating the known or predicted localization of individual SUMO1/SUMO2 conjugates. Top: All 1058 SUMO1/SUMO2 conjugates detected in wild-type, siz1-2, and mms21-1 plants ± heat stress (HS) at 37°C for 30 min. Middle: The 454 abundant SUMO1/SUMO2 conjugates detection in three or more biological replicates in the siz1-2 and/or wild-type backgrounds upon heat stress. Bottom: The 112 SUMO1/SUMO2 conjugates that were significantly increased/decreased in siz1-2 versus wild-type plants after HS.
(B) GO functional enrichment of all 922 SUMO1/SUMO2 conjugates that appeared during HS in wild-type and siz1-2 seedlings (green) and the 112 SUMO1/SUMO2 conjugates that accumulated during HS and appear to be SIZ1-influenced (orange).
(C) GO functional enrichment of the SIZ1-dependent SUMO1/SUMO2 targets compared with the total collection of 922 SUMO1/SUMO2 targets identified in the wild type and siz1-2 after HS.