Figure 2.

Subcellular Localization and Expression Pattern of STRK1.

(A) Plasma membrane localization of STRK1-YFP anchored by palmitoylation. Amino acid sequence of the N-terminal region of STRK1 showed the three cysteine residues (bold) as putative palmitoylation sites. Confocal images showed localization of STRK1-YFP or STRK1-C5,10,14A-YFP in rice protoplasts. Bar = 10 µm.

(B) to (H) STRK1 promoter-GUS expression patterns in transgenic rice plants. GUS expression was observed in young root ([B] and [C]), leaf vein (D), 4-d-old seedling (E), stem (F), leaf sheath (G), and young spikelet (H).

(I) and (J) Relative mRNA levels of STRK1 by RT-qPCR in the root (I) and shoot (J) of three-leaf stage rice seedlings treated with 150 mM NaCl and 1% (v/v) H₂O₂. Data are presented as mean ± sd (n = 3).