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. 2018 May 10;596(12):2283–2299. doi: 10.1113/JP275602

Figure 6. The glucose transport step is impaired in Rac1 mKO after high‐fat feeding.

Figure 6

A, basal and insulin (60 nm, 20 min) 2‐deoxy‐glucose (2DG) transport in soleus and extensor digitorum longus (EDL) muscle from wild type (WT) and muscle‐specific Rac1 knockout (mKO) mice following 18 weeks 60% high‐fat diet (HFD). B, insulin‐stimulated increase above basal of 2DG transport in soleus and EDL muscle. Basal and insulin‐stimulated p‐AktSer473 (C), p‐TBC1D4Thr642 (D), and Rac1 and GLUT4 protein expression (E), and representative western blots (F). Shaded areas indicate HFD. Significant effect of insulin stimulation is indicated by * (P < 0.001). Significant effect of Rac1 mKO is indicated by # (P < 0.001). n = 7–10. Two‐way repeated measures ANOVA was performed for each muscle. Fold changes were analysed using t tests. Values are means ± SD.