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. 2018 May 10;596(12):2283–2299. doi: 10.1113/JP275602

Figure 7. High fat feeding alter β‐actin mRNA (muscle) and protein content (adipose tissue) in mice.

Figure 7

Total expression of β‐actin protein (right bars) and gene expression in gastrocnemius muscle (A) and white adipose tissue (B) from wild type (WT) and muscle‐specific Rac1 knockout (mKO) on chow or 60% high‐fat diet (HFD) of glucose transporter 4 (glut4), forkhead box protein O1 (foxo1), peroxisome proliferator‐activated receptor γ (pparγ), interleukin 6 (IL‐6), tumour necrosis factor α (TNFα), F4/80, monocyte chemoattractant protein‐1 (mcp‐1), and β actin related to OliGreen (single‐stranded (ss) DNA). n = 8–10, basal and insulin‐stimulated samples pooled for each genotype (no effect of insulin was observed). Significant effect of diet is indicated by * (P < 0.05). Significant effect of Rac1 mKO is indicated by # (P < 0.05). Two‐way ANOVAs were performed for each gene. Values are means ± SD.