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. 2018 May 20;596(12):2345–2358. doi: 10.1113/JP275806

Table 1.

Primer sequences designed and validated for quantitative real‐time RT‐PCR analysis of fibrosis

Gene Primer sequence, 5′–3′ Accession number
COL1A
  • FWD‐ TACCATGACCGAGACGTGTGGAAA

  • REV‐ AGTGGTTTCTTGGTCCGTGGTTGA

AF129287
COL3A
  • FWD‐ AACCAGAACCGTGCCAAATATGCG

  • REV‐ TGGGCAAACTGCACAACATTCTCC

NM_001076831
TIMP1
  • FWD‐ ACTCCGAAGTCGTCATCAGG

  • REV‐ CCAGCAGCATAGGTCTTGGT

NM_001009319.2
TIMP2
  • FWD‐ AGATGGGCTGTGAGTGCAAGATCA

  • REV‐ AGAACTTGGCCTGATGTCCGTTGA

NM_001166186
TIMP3
  • FWD‐ CCTCTCCCAGCGCAAGGGGT

  • REV‐ GCCACCCTTCTGCCGGATGC

NM_001166187.1

FWD, forward; REV, reverse.