Figure 5.

Defective indirect presentation by bone marrow antigen‐presenting cells (BM‐APCs) abrogates insulin promoter‐driven hen egg lysozyme transgene (insHEL)‐mediated deletion and delays thyroglobulin promoter‐driven hen egg lysozyme transgene (thyroHEL)‐mediated deletion. Irradiated none‐transgenic (Non‐Tg), insHEL or thyroHEL hosts were reconstituted with 3A9 BM that was either wild‐type (H2‐Aa ^+/+) or MHCII‐deficient (H2‐Aa ^–/–) and analysed 4–6 weeks later. (a) Thymocytes were gated for TCR^3A9+ Foxp3⁺ and TCR^3A9+ Foxp3^– subsets (left) and the summary shows the number of TCR^3A9+ Foxp3⁺ thymocytes (right). (b) TCR^3A9+ Foxp3^– thymocytes were analysed for CD4/CD8 expression (left) and summaries show the number of double‐positive (DP) and CD4 single‐positive (CD4SP) cells (right). (c) Helios expression on DP and CD4SP subsets of TCR^3A9+ Foxp3^– thymocytes (left) with Helios relative fluorescence intensity (RFI) values relative to Non‐Tg DP thymocytes (right). Statistical comparisons used two‐way analysis of variance and Sidak's multiple comparisons test. P‐value symbols: *P < 0·05; **P < 0·01; ***P < 0·001; ****P < 0·0001.