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. 2018 Jun 14;8:9137. doi: 10.1038/s41598-018-27505-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Global gene expression variability of histologically normal breast tissue. (A) Global gene expression variation at different levels. Y-axis is variation-by-distance (VD). Greater distance indicates higher variation. Inter-individual variability, n = 8 for tumor and n = 184 for normal; intra-individual variability, n = 49 for inter-block, n = 20 for inter-section, and n = 7 for technical duplicates. (B) Contribution of block and section to overall expression variation of genes. X- and Y-axes are percentage of contribution of block and section to the global gene expression variation, respectively. Each dot represents one gene. The diagonal solid line indicates the ideal situation where contribution of block and section is equal.