Fig. 5.

SLC7A5 is required for NK cell metabolic and functional responses. a–i NK cells were stimulated with IL-2/IL-12 in the presence or absence of BCH (25 mM) for 18 h as indicated. a FSC-A and CD71 expression were analysed by flow cytometry. b, c Analysis of extracellular acidification rate (ECAR) of NK cells to assess basal glycolytic rate and glycolytic capacity. d, e Analysis of NK cell oxygen consumption rate (OCR) to assess rates of OXPHOS and maximal respiration. f, g IFNγ and h, i granzyme B expression were analysed by flow cytometry. Data are mean ± s.e.m. of 4 (c, e) or 5 (g, i) experiments or representative of 4 (b, d) or 5 (a–h) individual experiments. b–e Data were normalised to 200,000 cells. Statistical analysis was performed using Student's t-test (c–g) or a one-sample t-test vs. a theoretical value of 1 (i); *p < 0.05, **p < 0.01. Oligo oligomycin, 2DG 2-deoxyglucose, Anti A antimycin A, Rot rotenone, FCCP carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone