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. 2017 Dec 18;11(1):116–124. doi: 10.1016/j.tranon.2017.10.003

Figure 3.

Figure 3

ADH7 – marker for Barrett‘s and Gastric mucosa? Reduced expression of retinol metabolic enzyme ADH7 was found in esophageal adenocarcinoma (EAC) but also in Barrett's esophagus (BE) and already in metaplastic gastric epithelium (GE) of the cardia. Data obtained by microarray analysis (left side) are in agreement with those of RT-PCR of an independent set of samples. Left side: mRNA expression of ADH7 in the training cohort determined by microarray analysis. Gene expression is significantly differential expressed between groups (corrected P-value <0.0001). Data analysis was performed on 18 Healthy, 20 BE and 21 EAC samples. The horizontal axis depicts the three patient groups: Healthy, BE and EAC. Gene expression is presented as normalized (log2 scale) signal intensity. Right side: Relative mRNA expression of ADH7 in the validation cohort. The mRNA expression was examined in 12 samples from Healthy tissue, 20 BE, 8 GE and 10 EAC by means of quantitative real-time PCR. Quantitation is relative to the transcription of GAPDH. Significance in differential expression of individual gene between groups was calculated using Kruskal-Wallis test (P-value <0.001). The horizontal axis shows four patient groups: Healthy, BE, GE and EAC.