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. 2018 Jun 14;8:9158. doi: 10.1038/s41598-018-26746-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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NXT ameliorates glucose metabolism in db/db mouse tissues. After treatment, liver, skeleton muscle and white adipose tissue were collected from db/db mice followed by total cellular protein extraction. Expression of PCK1, G6Pase, GCK, AMPKα, pi-AMPKα and FGF21 in the liver (a,b, upper panel of d), GLUT4 in the skeleton muscle (c), and FGF21 in the white adipose tissue (lower panel of d) was determined by Western blot. *p < 0.05 vs. db/db control groups (n ≥ 5).