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. 2018 Jun 14;8:9101. doi: 10.1038/s41598-018-27414-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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WNK1 silencing increased FOXO4 nuclear localization in C2C12 cells. Immunoblots (left) and densitometric analyses (right) of three FOXO isoforms, namely FOXO1, FOXO3, and FOXO4, as well as Smad2/3 after separating nuclear (upper)/cytoplasmic (lower) extracts in WNK1-silenced C2C12 cells. FOXO4 levels were selectively increased in the nuclei of C2C12 cells treated with WNK1-targeted siRNA. The hash (#) shows FOXO4. Values are presented as the mean ± standard error of the mean (n = 4 or 7 per experimental group). *P < 0.05 versus the control cells. Full-length blots are presented in Supplementary Fig. 9.