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. 2018 Jun 14;6:19. doi: 10.1038/s41413-018-0021-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Lineage tracing of chondrocytes to osteoblasts. a The lineage trace construct consists of a tamoxifen-inducible CRE, which is driven by a promoter of a chondrogenic tissue, such as Sox9, Col2, Col10, or Acan. This in turn excises the stop codon blocking the ROSA-LSL-TdTomato construct. All further cells in the recombined lineage will express td-tomato. b, b′ ROSA-LSL-TdTomato floxed mice, positive for Col2-Cre^ERT2, were administered with tamoxifen at P3 and mice euthanized at P10. Coexpression of OSX and Tomato-red in cells of the primary spongiosa. c, c′ Coexpression of OSX and Tomato-red in cells of the epiphysis. d, d′ Coexpression of COL1 and Tomato-red in cells of the primary spongiosa. e, e′ Coexpression of COL1 and Tomato-red in cells of the epiphysis. COL1, collagen type 1; OSX, osterix; TOM, Tomato-red; DAPI, nuclear stain; PS, primary spongiosa; GP, growth plate; EP, epiphysis.