Skip to main content
. 2018 Jun 8;9:308. doi: 10.3389/fendo.2018.00308

Figure 1.

Figure 1

Phylogenetic analysis and tissue distribution of ERs and protein-coupled estrogen receptors (GPERs) in grass carp. (A) Phylogenetic analysis of ER amino acid sequences in vertebrate species using neighbor-joining method with MEGA6.0. The Oryzias latipes AR was used as the outgroup. (B) Phylogenetic tree of grass carp GPER using predicted amino acid sequences using neighbor-joining method with MEGA6.0 program. NK3R from grass carp served as an outgroup to root the tree. (C) Tissue distribution of estrogen receptor alpha (ERα), estrogen receptor beta 1 (ERβ1), estrogen receptor beta 2 (ERβ2), GPER1a, and GPER1b in grass carp brain areas. Total RNA was isolated from brain areas in grass carp and subjected to reverse transcription PCR (RT-PCR) using primers specific for carp ERα, ERβ1, ERβ2, GPER1a, and GPER1b transcripts, respectively. Parallel RT-PCR for β-actin was also conducted to serve as the internal control.