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. 2018 Jun 8;9:308. doi: 10.3389/fendo.2018.00308

Figure 2.

Figure 2

E2 induce luteinizing hormone (LH) secretion and mRNA expression in grass carp pituitary cells. (A) In the time-course experiment, pituitary cells were incubated with E2 (10 nM) for the duration as indicated. (B) In parallel studies for dose dependence, pituitary cells were challenged for 24 h with increasing levels of E2 (1–1,000 nM). (C) Effects of E2 (10 nM), sGnRH (1 µM), and cGnRH (1 µM) on LH and follicle-stimulating hormone (FSH) mRNA expression in grass carp pituitary cells. After drug treatment, culture medium was harvested for measurement of LH secretion, and the remaining cells were used for total RNA preparation for real-time PCR of the LHβ and FSHβ mRNA. Data presented are expressed as mean ± SEM, and groups denoted by different letters represent a significant difference at P < 0.05 (ANOVA followed by a Dunnett’s test).