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. 2018 Jun 8;9:308. doi: 10.3389/fendo.2018.00308

Figure 4.

Figure 4

Receptor specificity of E2-induced luteinizing hormone (LH) secretion and mRNA expression. (A) Effects of ER agonists on LH transcript expression and hormone secretion. For LHβ mRNA expression, pituitary cells were treated for 24 h with E2 (10 nM), G protein-coupled estrogen receptor (GPER) agonist G-1 (1 µM), estrogen receptor alpha agonist propylpyrazole triol (PPT) (1 µM), and ERβ agonist DPN (1 µM), respectively. (B) Effects of GPER antagonist G-15 and ER antagonist ICI182780 on E2-induced LHβ secretion and mRNA expression. Pituitary cells were treated for 24 h with E2 (10 nM) in the presence or absence of the G-15 (10 µM) or ICI182780 (10 µM), respectively. (C) Effects of GPER antagonist G-15 or ER antagonist ICI182780 on G-1- or DPN-induced LH secretion and mRNA expression. Grass carp pituitary cells were incubated for 24 h with GPER agonist G1 (1 µM) in the presence or absence of G-15 (10 µM), or ERβ agonist DPN (1 µM) in the presence or absence of ICI182780 (10 µM).