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. 2018 Jun 8;9:308. doi: 10.3389/fendo.2018.00308

Figure 7.

Figure 7

Signal transduction of E2-induced luteinizing hormone (LH)β and follicle-stimulating hormone (FSH)β mRNA expression in grass carp pituitary cells. (A) Effects of 24-h co-treatment with the adenylyl cyclase inhibitor MDL12330A (20 µM) or protein kinase A inhibitor (20 µM) on E2 (10 nM)-induced LHβ and FSHβ mRNA expression. (B) Effects of 24-h co-treatment with the phospholipase C inhibitor U73122 (10 µM) or protein kinase C inhibitor GF109203X (20 µM) on E2 (10 nM)-induced LHβ and FSHβ mRNA expression in carp pituitary cells. (C) Effects of 24-h co-treatment with inositol 1,4,5-triphosphate receptor blocker 2-APB (100 µM) or VSCC blocker Nifedipine (10 µM) on E2 (10 nM)-induced LHβ and FSHβ mRNA expression. (D) Effects of 24-h co-treatment with calmodulin antagonist calmidazolium (1 µM) or CaM-dependent protein kinase II inactivator KN62 (10 µM), respectively, on E2 (10 nM)-induced LHβ and FSHβ mRNA expression in grass carp pituitary cells.