Table 2.

Characteristics of the PCR-based human papillomavirus (HPV) assays.

HPV detection method	HPV-genomic regions targeted by the primer sets	Amplicon size	Included controls	HPV genotypes detected	LODa
SPF10LiPA25 (strip-based reverse hybridization)	L1	65 bp	External:	HPV 6, 11, 16, 18, 31, 33, 34, 35, 39, 40, 42, 43, 44, 45, 51, 52, 53, 54, 56, 58, 59, 66, 68, 70, and 74	1:100.000b
			SiHa cells
			Pure paraffin samples
			Cytolomegavirus (CMV) control
			HPV 18 positive and HPV-negative PCR control
			HPV-positive + borderline + negative DNA enzyme immunoassay control
			Internal:
			None
Semi-quantitative- PCR (semi-Q-PCR)	E6/E7	82–134 bp	External:	HPV 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, and 59	Not measured
			CMV control
			Pure paraffin sample
			HPV-positive control for the specific HPV genotype tested for in each Q-PCR
			Internal:
			Glyceraldehyde-3-phosphate dehydrogenase (DNA control)

^aLimit of detection.

^bBased on dilutions of SiHA cell lines, which contain 1–2 HPV 16 copies per cell line.