Fig. 4.

Kif3b is required for collagen fiber alignment at the tumor front in vivo. a Primary tumor fronts of HEp3 control (upper panel) and HEp3 shRNA2 Kif3b (lower panel) tumors as visualized using intravital confocal microscopy. b Collagen fiber organization (SHG) along the HEp3 control (upper panel) and shRNA2 HEp3 Kif3b (lower panel) tumor fronts, see also Supplementary Movie 5. c Higher magnification images of the collagen fiber network from within white dashed rectangles in (b). Yellow dashed lines delineate tumor borders. d Quantification of collagen bundle density at the invasive fronts of control and shRNAs1/2 Kif3b HEp3 tumors. e Quantification of collagen fiber alignment at the invasive fronts of control and shRNAs1/2 Kif3b HEp3 tumors. f Quantification of cancer cell protrusion orientation at the invasive fronts of control and shRNAs1/2 Kif3b HEp3 tumors. Scale bars = 100 μm