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. 2018 Jun 14;8:9105. doi: 10.1038/s41598-018-27358-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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G6PD expression and activity are suppressed by miR-122 and miR-1 in HepG2 cells. HepG2 cells transfected with scrambled miRNA (NC), miR-1, miR-122, combo (miR-1 and miR-122), G6PD siRNA (siG6PD) or negative control siRNA (NC) for 48 hours. (a) G6PD activities in these cells were determined by measuring NADPH absorbance at 341 nm and calculating the NADP⁺/NADPH ratios. Asterisks indicates p-value < 0.05, p-value was calculated using two-tailed Student T-test). (b) Immunoblotting of G6PD protein was performed in the lysates. G6PD levels were normalized to that of vinculin. (c,d) Cell viability measured using CellTiter-Glo® Luminescent kit. Cell viability over time was determined as change in luminescence from 0-hour time point. Cropped immunoblot images in (b) were obtained from the same gel. Full immunoblot images are found in supplemental material.