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. 2018 Jun 14;8:9128. doi: 10.1038/s41598-018-27529-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Frequency distribution curves of B. subtilis and B. cereus spores treated at sub-inhibitory concentrations of C1, C2, C3, C4 and C5 (red line). The start of germination and germination time are depicted. Treated conditions were overlaid with the untreated B. subtilis and B. cereus spores (black line). The histogram was normalized to occupy an area of one and was rescaled so that the maximum value in the histogram is equal to one. Significance of differences between the median values of the two groups was assessed using the Mann-Whitney test. Observations of two biological repeats were grouped and analysed as one data set. See Table 3 for the concentrations used.