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. 2018 Jun 14;8:9128. doi: 10.1038/s41598-018-27529-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Snapshots of the live imaging results of B. subtilis spores untreated and treated with sub-inhibitory concentrations of C1, C3 and C4. Absolute time of the live cell imaging are shown from the start of sample preparation. Treatment with C1 show the start of cell wall damage 3 hrs and 54 min after the start of measuring, but cell lyse only occur 2.5 hrs after spore bursting (red arrows). Treatment with C3 resulted in enlarged cells that appeared to be swelling directly after bursting. The deformed cells appear to not divide, but elongate into a cell with irregular curvatures. Treatment with C4 also resulted in cell lysis 2.5 hrs after spore bursting (red arrows). Scale bar represent 2 µm.