Figure 2.

BnAbs Are Enriched For Improbable Antibody Mutations

(A) Table of improbable mutations (at the < 2% cutoff level) for a representative set of bnAbs. (B) Distributions of the number of improbable mutations from antibody heavy-chain sequences from three groups: (1) “RV144-induced” antibodies were isolated from RV144 vaccinated subjects by antigenically sorting with RV144 immunogens (red shaded area); (2) “uninfected” antibodies correspond to duplicated NGS reads from IgG antibodies isolated from PBMC samples from 8 HIV-uninfected individuals (blue shaded area; see methods for details on sampling); and (3) a representative set of published bnAbs are shown labeled above dotted lines that correspond to their number of improbable mutations. Green labels indicate bnAbs with high potency (< 1 ug/mL) and breadth (> 90%).