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. 2018 Jun 8;9:1188. doi: 10.3389/fmicb.2018.01188

Table 1.

Description of the crops and the treatments applied to the fields.

2008–2013 2014
Treatment Crop rotationa Cropb Seeding density (seeds m−2)c Nutrient supply (%)d Lacombe, AB Brandon, MB Beaverlodge, AB
Actual N-P-K-S (Kg/Ha)
Can_REe Cr-Cr-Cr-Cr-Cr-Cr InVigor L135C 100 100 115-46-36-0 89-34-0-10 117-25-0-0
Can_HFe Cl-Cl-Cl-Cl-Cl-Cr InVigor L135C 100 150 174-54-54-17 125-34-0-10 193-41-16-16
Can_HDe Cf-Cf-Cr-Cf-Cf-Cr InVigor L135C 150 100 115-46-36-0 89-34-0-10 117-25-0-0
Wheat W-Cl-Cr-W-Cl-Cr Stettler Wheat 300 100 134-46-36-0 134-51-0-14 131-25-0-0
Pea P-W-Cr-P-W-Cr CDC Meadow Pea 100 100 10-46-36-0 7-34-0-0 6-28-0-0
a

Cr, canola possessing the gene for resistance to glyphosate; Cl, canola possessing the gene for resistance to glufosinate herbicides; Cf, canola possessing the gene for resistance to imidazolinone.

b

InVigor L135C is a Cl canola cultivar. The seeds were treated by Prosper Evergol, which is a combination of Clothianidin (insecticide component), Penflufen, Trifloxystrobin and Metalaxyl (3 fungicides components).

c

Seeding was performed with air seeders equipped with knife openers, and crops were seeded at optimal depths in 20- to 30-cm rows. Plot dimensions were 3.7 × 15.2 m.

d

Fertilization (N, P2O5, K2O, and S) was based on soil tests and adjusted to achieve 100 or 150% of the recommendations for each crop species. Nitrogen was added as ESN Smart Nitrogen (Agrium Inc., Calgary, Alberta, Canada).

e

Canola grown as recommended (Can_RE), canola fertilized at 150% of the recommended rate (Can_HF), and canola seeded at 150% of the recommended rate (Can_HD).