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. 2018 Jun;58(6):756–766. doi: 10.1165/rcmb.2017-0236OC

Figure 2.

Figure 2.

CRIg is expressed in the lung. (A) Western blot detection of mouse TRIM72 (mTRIM72) and mouse CRIg (mCRIg; ∼32 kD) in primary alveolar macrophages (AMs) from the wild-type (WT) and TRIM72KO (T72KO) mice. (B) CRIg and TRIM72 were detected in developing mouse lungs from Postnatal Day 1 to Postnatal Day 30 (d1–d30) by Western blot; levels of β-actin (ACTB) were used as loading controls. (C) Detection of endogenous CRIg in human (h) lungs (long form, ∼55 kD). (D) TRIM72 and CRIg were detected in WT AMs at resting stage, or exposed to Pseudomonas aeruginosa (P.a.) for 60 minutes. (E) immunostaining detection of TRIM72 (green) and CRIg (magenta) in primary human AMs. Cell was costained with DAPI (blue), and negative control using IgG only was included. Experiments were repeated three times.