Figure 6.

TRIM72 ablation improves bacterial clearance and survival in P.a. pneumonia. (A) Percent body weight (B.W.) loss of naive WT and TRIM72^KO mice after the first intraperitoneal injection of roughly 2.5 × 10⁵ cfu/ml PAO1 (a clinical isolate of P.a.); n = 13 for WT (black squares), n = 8 for TRIM72^KO (red circles), *P < 0.05, **P < 0.005 compared with WT. (B) Percentage of survival at Day 2 after roughly 3 × 10⁷ cfu/ml P.a. intraperitoneal injection; n = 10 for WT (solid black squares) and TRIM72^KO (solid red circles), n = 6 for adoptive transfer of WT AM to TRIM72^KO (open black squares) and for KO AM to TRIM72^KO (open red circles); *P < 0.05 for WT versus TRIM72^KO groups, and for WT AM to TRIM72^KO versus KO AM to TRIM72^KO groups. (C) Scatter plot of whole-lung bacterial burden at Day 2 P.a. injection in WT, TRIM72^KO, WT AM to TRIM72^KO, and KO AM to TRIM72^KO groups. Gray dashed line designates injected bacterial dose; ^ designates mice that have died. P < 0.05 for WT versus TRIM72^KO groups, and for WT AM to TRIM72^KO versus KO AM to TRIM72^KO, n = 6–10 (as described in B). (D) At Day 2 after P.a. injection, ELISA detection of cytokine levels of TNF-α, IL-6, IL-1β, IL-12, and IL-10 in BALF; total cell number in BALF was measured by hemocytometer counting, n = 5 for WT PBS (open bar), n = 10 for WT P.a. (open bar), and n = 9 for TRIM72^KO P.a. (solid bar), *P < 0.05, **P < 0.005 compared with WT PBS; ^#P < 0.05, ^##P < 0.005 compared with WT P.a. (E) Lung tissue levels of TNF-α, IL-6, IL-1β, IL-12, and IL-10 in WT AM to TRIM72^KO and KO AM to TRIM72^KO groups at Day 2 after P.a. injection; n = 6, **P < 0.005. Data are presented as mean (±SE).