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. 2018 Jun;58(6):736–744. doi: 10.1165/rcmb.2017-0329OC

Figure 4.

Figure 4.

Neutrophil depletion in pIgR−/− mice blocks small airway wall fibrosis and emphysema. pIgR−/− mice were treated with anti-Ly6G or anti-IgG2a isotype control antibodies between 4 and 8 months of age. (A) Quantification of parenchymal neutrophil numbers after immunostaining lung sections with neutrophil elastase–specific antibodies; n = 8–9 mice/group; *P < 0.001 (t test). (B) Western blot and (C) densitometry for neutrophil elastase (NE; 26 kD) in lung tissue, normalized to β-actin; n = 6–7 mice/group; *P < 0.001 (t test). (D) Elastase activity in whole-lung lysates; n = 4–5 mice/group; *P < 0.05 (t test). (E) Morphometric analysis of small airway wall thickness (VVairway); n = 8–9 mice/group; *P < 0.01 (t test). (F) Morphometric analysis of emphysema (mean alveolar septal perimeter); n = 8–9 mice/group; *P < 0.0001 (t test).