FIG 3.

Inhibition of uc.173 impairs gut barrier function in mice subjected to CLP. (A) Levels of uc.173 (left), uc.346 (center), and U6 (right) RNAs in the small intestinal mucosae of mice injected intraperitoneally with LNA-modified anti-uc.173 or control oligonucleotides (Con-oligo) for 4 consecutive days. On day 5, total RNA was isolated for RT-Q-PCR analysis. Values are relative to those with Con-oligo and are means ± SEM of data from 5 animals. The asterisk indicates a significant difference (P < 0.05) from the Con-oligo result. (B) Gut permeability in sham-treated mice and in mice exposed to CLP for 24 h. FITC-dextran was given orally, and blood samples were collected 4 h later. The asterisk and plus sign indicate significant differences (P < 0.05) from sham-treated mice and from mice treated with Con-oligo and CLP, respectively. (C) Representative immunoblots of claudin-1, claudin-3, and β-catenin in the small intestinal mucosae of mice treated as described for panel B.