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. 2018 Jun 14;38(13):e00010-18. doi: 10.1128/MCB.00010-18

FIG 4.

FIG 4

uc.173 interacts with miR-29b but not with CLDN1 mRNA. (A) Schematic of plasmids used for assays of CLDN1 mRNA and miR-29b pulldown. pMS2 and pMS2-uc.173 expressed MS2 and MS2-uc.173 RNAs, respectively, each containing 24 tandem MS2 hairpins; pMS2-GST expressed a fusion protein (MS2-GST) that can pull down MS2-containing RNA. (B) Levels of uc.173 (a) and MS2-BP (b) 24 h after cotransfection with pMS2-GST and the pMS2 or pMS2-uc.173 vector. Values are relative to those with the pMS2 vector and are means ± SEM from triplicate experiments. The asterisk indicates a significant difference (P < 0.05) from the result for cells transfected with pMS2. (C) Binding of pMS2-uc.173 to RNAs. Shown are levels of CLDN1 mRNA and miR-29b in the materials pulled down by pMS2-uc.173, given relative to levels with pMS2 (a) and as percentages of total input RNAs (b). The asterisk indicates a significant difference (P < 0.05) from the result for cells transfected with pMS2.