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. 2018 Jun 14;38(13):e00010-18. doi: 10.1128/MCB.00010-18

FIG 6.

FIG 6

miR-29b represses claudin-1 translation. (A) Schematic representation of CLDN1 mRNA depicting predicted target sites for miR-29b in its 3′ UTR. BS, predicted miR-29b-binding site. (B) Binding of biotinylated miR-29b to CLDN1 mRNA. (a) Schematic representation of biotinylated miR-29b; (b) miR-29b levels 24 h after transfection; (c) levels of mRNAs encoding claudin-1, E-cadherin (E-cad), and CUG-binding protein 1 (Cugbp1) in materials pulled down by biotin–miR-29b. Values are relative to that with the control scrambled oligomer and are means ± SEM of data from triplicate experiments. The asterisk indicates a significant difference (P < 0.05) from the result for cells transfected with the control scrambled oligomer. (C) Levels of miR-29b in cells transfected with pre-miR-29b for 48 h. The asterisk indicates a significant difference (P < 0.05) from the result for cells transfected with the control scrambled oligomer. (D) Representative immunoblots of claudin-1, E-cadherin, and β-catenin in cells treated as described for panel C. (E) Distribution of CLDN1 mRNA in each gradient fraction prepared from polysomal profiles in cells treated as described for panel C. The levels of CLDN1 or GAPDH mRNAs were measured and plotted as percentages of total CLDN1 or GAPDH mRNA levels in the samples, respectively.