FIG 5.

Insulin regulates myelin protein P₀ level in an EGR2-dependent manner. (A) The effect of insulin on Mpz expression in primary Schwann cells from both wt and Dhtkd1^−/− mice. (B) The effect of insulin on Egr2 mRNA expression. (C) Real-time PCR analysis of the expression patterns of other myelin genes after insulin treatment. (D and E) P₀ expression decreased with the increase of insulin concentrations, as assayed via immunofluorescence (D) and Western blotting (E). (F) Real-time PCR analysis of the expression S100b, Bdnf, and Ngf. A Student two-sided t test was used for data in panels A to C and F, and values are shown as means ± SD. *, P < 0.05; **, P < 0.01.