FIG 5.

Ultrastructural analysis of HSV-2-infected cells. Vero cells were infected with HSV-2 SD90e (A and B) and the UL16 deletion mutant, UL16SΔ10-360 (C and D), at an MOI of 3. At 16 hpi, cells were fixed and processed for TEM as described in Materials and Methods. (B) Nonenveloped cytoplasmic capsids and enveloped virions can be observed in the cytoplasm of SD90e-infected Vero cells. (D) These structures were rarely observed in the cytoplasm of UL16SΔ10-360-infected cells. (A and C) Nuclear capsids were readily detected in the nuclei of SD90e-infected (A) and UL16SΔ10-360-infected (C) cells. (Insets) Magnified portions of the images. (A) The white arrowhead identifies an A capsid, whereas a B capsid is identified with a black arrow and a C capsid with a black arrowhead. (B) The black arrow indicates an enveloped capsid and the black arrowhead a nonenveloped capsid.