TABLE 1.
Quantification of intracellular capsids in Vero cells infected with UL16 mutant and parental HSV strainsa
| Strain | No. (%) of capsids |
|||||
|---|---|---|---|---|---|---|
| Total | Intranuclear |
Cytoplasmic |
PNSb | |||
| A + B | C | Nonenveloped | Enveloped | |||
| SD90e | 948 | 377 (39.8) | 96 (10.1) | 250 (26.4) | 53 (5.6) | 172 (18.1) |
| UL16SΔ10-360 | 992 | 677 (68.2) | 75 (7.6) | 159 (16) | 13 (1.3) | 68 (6.9) |
| HG52 | 1,429 | 676 (47.3) | 107 (7.5) | 441 (30.9) | 163 (11.4) | 42 (2.9) |
| UL16HΔ10-360 | 990 | 702 (70.9) | 91 (9.2) | 156 (15.7) | 29 (2.9) | 12 (1.2) |
| KOS | 850 | 249 (29.3) | 75 (8.8) | 296 (34.8) | 171 (20.1) | 59 (6.9) |
| UL16KΔ28-359 | 1,058 | 506 (47.8) | 58 (5.5) | 452 (42.8) | 35 (3.3) | 7 (0.7) |
| F | 663 | 217 (32.7) | 77 (11.6) | 175 (26.4) | 126 (19) | 68 (10.3) |
| UL16FΔ139-359 | 1,778 | 892 (50.2) | 84 (4.7) | 671 (37.7) | 99 (5.6) | 32 (1.8) |
a
Capsids were counted in different cellular compartments from 10 images/strain derived from multiple sections in two independent experiments.
b
PNS, perinuclear space.