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. 2018 Jun 13;92(13):e00368-18. doi: 10.1128/JVI.00368-18

FIG 5.

FIG 5

Generation of fluorescently labeled HIV-1. (A) Schematic design of the Vpr-mRuby3-IN construct used to label HIV-1 particles in trans. (B) Specific infectivity (luciferase per nanogram of p24) was measured for D116N HIV-1 complemented in trans with no plasmid, Vpr-RT, Vpr-IN, or Vpr-mRuby3-IN. (C) TIRF image of WT HIV-1 labeled with Vpr-mRuby3-IN. (D) Confocal image of HeLa cells synchronously infected with WT HIV-1 labeled with Vpr-mRuby3-IN and 5-ethynl uridine and fixed 30 min postinfection.