TABLE 1.
Estimated molecular masses of the CPSF6-358 proteins from the c(s) analysisa
| Protein | Monomer mass (kDa)b | Concn (mg/ml) | Major species |
f/f0d | |||
|---|---|---|---|---|---|---|---|
| Peak 1 |
Peak 2 |
||||||
| Mass (kDa) | Sedimentation coefficient (S) | Mass (kDa) | Sedimentation coefficient (S) | ||||
| H6–albumin–CPSF6-358 P1 | 110.0 | 1.0 | 56.4 | 5.4 | 117.9 | 8.8 | 0.98c |
| 0.5 | 98.4 | 5.0 | 217.3 | 8.4 | 1.55 | ||
| 0.25 | 125.1 | 5.4 | 275.2 | 9.2 | 1.66 | ||
| H6–albumin–CPSF6-358 P2 | 110.0 | 1.0 | 88.1 | 4.9 | 141.4 | 6.7 | 1.46 |
| 0.5 | 88.7 | 5.0 | 152.3 | 7.1 | 1.45 | ||
| 0.25 | 102.7 | 5.1 | 216.8 | 8.3 | 1.56 | ||
| CPSF6-358 | 37.8 | 1.0 | 89.0 | 4.3 | 1.68 | ||
| 0.5 | 89.6 | 4.3 | 1.68 | ||||
| 0.25 | 65.2 | 4.2 | 1.40 | ||||
a
For each sample concentration, the signal-weighted sedimentation coefficient and the estimated molecular mass of each species are shown, together with the best-fit frictional ratio for the distribution.
b
Samples also include an amount of aggregated material.
c
Frictional ratios of <1.0 are not possible and do not actually have any physical meaning. This value reflects the software's attempt to fit such a diverse range of species.
d
Frictional ratio parameter.