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. 2018 Jun 13;92(13):e02116-17. doi: 10.1128/JVI.02116-17

FIG 6.

FIG 6

OPN interacts with HCV proteins. (A) Equal amounts of cellular lysates from mock- and HCV-infected Huh7.5 cells were immunoprecipitated with anti-OPN (1:100 dilution) and immunoblotted with anti-NS5A, anti-NS5B, anti-NS3, and anti-core antibodies (lanes 1 and 2). Isotype anti-goat IgG was used as a control (lane 3). (B) Cellular lysates from Huh7 and K2040 cells were immunoprecipitated using anti-OPN and Western blotted (WB) with the above-named HCV antibodies (lanes 1 and 3), including isotype control goat IgG (lane 2). Cellular lysates from HCV-infected cells and K2040 cells were utilized to perform reverse co-IP using anti-NS3 and anti-core antibodies and immunoblotted with anti-OPN (A and B). Right panels show the input controls.