FIG 6.

Mapping of tier 2 neutralizing antibody responses. (A) Purified polyclonal IgGs from vaccinated guinea pigs were evaluated against the tier 2 pseudovirus JRCSF. Horizontal black lines indicate mean titers, gray dots indicate responses below the limit of detection of the assay, and blue and black dots represent responses that were both detectable and greater than the limit of detection. Each dot represents the result for a single guinea pig. (B to D) Mapping was conducted with CD4 binding site competition by RSC3 (B), on a V3 glycan knockout virus (S334A) (C), and with V3 peptide competition (D). Tables show neutralization potencies against wild-type virus compared to mutant virus or against a virus with and without competition. +, increased neutralization potency; −, decreased neutralization potency.