FIG 13.
BHV-1 genomic structure and schematic map of VP22 and gM deletion plasmids (pBHV-1 VP22Δ GFP and pBHV-1 gMΔ GFP). The genomic organization of BHV-1 depicted at the top consists of unique long (UL) and unique short (US) regions and two repeat regions (internal repeat [IR] and terminal repeat [TR]). (A) Localizations of the UL49 gene (VP22) and its flanking UL50, UL49.5, and UL48 genes are shown. In plasmid pBHV-1 VP22Δ GFP, coding sequences for amino acids 34 to 149 of VP22 are deleted (nt 9499 to 9845) and two stop codons (uppercase residues in TAAcTGA) and KpnI and BamHI restriction sites are incorporated immediately downstream of the deletion site for the insertion of the eGFP gene cassette. (B) Localizations of the UL10 gene (gM) and its flanking UL12, UL11, and UL9 genes are shown. In plasmid pBHV-1 gMΔ GFP, nt 84819 to 85187 coding for gM amino acid residues 289 to 411 were deleted, and HindIII and KpnI restriction sites are incorporated at the deletion locus for the insertion of the eGFP gene cassette.