FIG 6.
Analysis of UL49.5 mutant viruses for UL49.5 and gM virion incorporation. Virions were partially purified through a 30% sucrose cushion and ultracentrifugation as described previously (31). Virion lysates of BHV-1 wt and various UL49.5 cysteine residue mutants were separated by a 5 to 20% gradient SDS-PAGE gel and immunoblotted with anti-UL49.5-specific (A) or anti-gM-specific (B) antibodies. Immunoblotting with anti-gC-specific antibody (C) served as a loading control. The 43-kDa mature and the 36-kDa immature gM proteins are shown. Note that in the case of UL49.5 C42S/CT-null virus, an approximately 43-kDa protein was detected by the gM-specific antibody; similar bands were not EndoH resistant (data not shown).