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Analysis of UL49.5 incorporation in the virion envelope of a gMΔ mutant virus. Partially purified virions and infected cell lysates of BHV-1 wt and gMΔ viruses were separated by SDS-PAGE and immunoblotted with anti-gM- or anti-UL49.5-specific antibodies. Immunoblotting with anti-gC-specific antibody served as loading control.
