TABLE 3.
Nucleotide and amino acid changes of BUNV passaged on Vero-ISG20 cellsa
| Segment | Position (nt) | Nucleotide mutation |
Amino acid mutation | Note | ||
|---|---|---|---|---|---|---|
| P0 | P20 | P40 | ||||
| L | 3,257 | G | G | A | Synonymous | |
| 5,267 | A | A | G | I1740M | L protein | |
| 6,538 | A | A/Gb | G | E2163A | L protein | |
| M | 518 | T | T/Ab | T | Synonymous | |
| 1,371 | G | G/Ab | G | V439I | NSm | |
| 3,568 | A | Gc | Gc | N1171S | Gc protein | |
| 4,388 | A | A/Gb | G | None | cRNA 3′ UTR | |
| S | 112 | C | C/Tb | T | Synonymous | N protein |
| S3L | NSs protein | |||||
| 330 | A | C | C | N82T | N protein | |
| T76P | NSs protein | |||||
a
The following differences were observed in the P0 virus relative to the sequence in GenBank: for the L segment (accession number NC_001925.1), T5501C (silent) mutation and 6,809-nucleotide C insertion in the 5′ UTR; for the M segment (accession number NC_001926.1), G415A (R120K), A417G (K121E), G1423A (R456K), A1941G (S629G), C2638T (P861L), G2829A (E925K), T3359C (silent), G3568A (S1171N), A4008G (R1381G), and G4317A (A1421T); and for the S segment (accession number NC_001927.1), not available.
b
Polymorphism.
c
Reversion to the GenBank sequence.