TABLE 4.
Nucleotide and amino acid changes of BUNV passaged on Vero-EMPTY cellsa
| Segment | Position (nt) | Nucleotide mutation |
Amino acid mutation | Note | ||
|---|---|---|---|---|---|---|
| P0 | P20 | P40 | ||||
| L | 3,299 | A | A | T | Synonymous | |
| 3,622 | A | A | A/Gb | E1191G | L protein | |
| M | 518 | T | T/Ab | T | Synonymous | |
| 1,371 | G | G/Ab | G | V439I | NSm | |
| 2,500 | C | T | T | P815L | Gc protein | |
| 4,240 | T | T/Ab | A | I1395N | Gc protein | |
| S | 84 | T | T | C | None | 3′ UTR |
| 118 | T | T | C | Synonymous | N protein | |
| L5P | NSs protein | |||||
| 165 | A | G | G | K27R | N protein | |
| S21G | NSs protein | |||||
a
The following differences were observed in the P0 virus relative to the sequence in GenBank: for the L segment (accession number NC_001925.1), T5501C (silent) mutation and 6,809-nucleotide C insertion in the 5′ UTR; for the M segment (accession number NC_001926.1), G415A (R120K), A417G (K121E), G1423A (R456K), A1941G (S629G), C2638T (P861L), G2829A (E925K), T3359C (silent), G3568A (S1171N), A4008G (R1381G), and G4317A (A1421T); and for the S segment (accession number NC_001927.1), not available.
b
Polymorphism.