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. 2018 Jun 13;92(13):e00092-18. doi: 10.1128/JVI.00092-18

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Copyright © 2018 DeLucia et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 4 — Number of DC-SIGN⁺ MΦ is lower in NP than in PR. (A) Gating strategy and live cell selection for MΦ phenotype by flow cytometry. (B) CD16 surface expression level (MFI) of SN CD14⁺ monocytes (day 0) and MΦ (day 7) was assessed by flow cytometry (n = 9). (C and D) Representative fluorescence intensity histograms (isotype, black; experimental, white) and individual MFI of MΦ measured by flow cytometry at day 7 for surface expression of CD4 and CCR5 (C) and CD209 (DC-SIGN) and CD169 (Siglec-1) (D). (E) Percentage of MΦ displaying surface expression of DC-SIGN and Siglec-1 was analyzed by flow cytometry and compared among SN (n = 9 or 10), NP (n = 5), and PR (n = 5). *, P ≤ 0.05. Histograms represent the means ± SE.